Figure 2

Detection of Ki-67, mutant P53 and VEGF expression in PTC lesions by immunohistochemical staining (400×). Formalin-fixed and paraffin-embedded PTC tissue sections were stained with the Elivision™ plus two-step system and specific antibodies against Ki-67, mutant P53 and VEGF. (A) PTC tissue stained with normal rabbit IgG instead of a specific primary antibody (negative control); (B) PTC tissue with positive Ki-67 staining (nuclear expression, total score 5); (C) PTC tissue with positive mutant P53 staining (combined nuclear and cytoplasmic expression, total score 7); (D) PTC tissue with positive VEGF staining (cytoplasmic expression, total score 7).