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Table 2 Primary and secondary antibodies for immunoblot analysis

From: Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein

Primary Antibodies
Antibody name Supplier Product number Source Dilution Molecular weights
Akt-1 Cell Signaling 2938 Rabbit 1:1000 60 kDa
Akt-2 Cell Signaling 5239 Mouse 1:1000 60 kDa
Akt-3 Cell Signaling 3788 Rabbit 1:1000 60 kDa
p-Akt Cell Signaling 4060 Rabbit 1:2000 60 kDa
Cleaved Caspase-3 Cell Signaling 9661 Rabbit 1:1000 17/19 kDa
Cleaved Caspase-9 Cell Signaling 9507 Rabbit 1:1000 17 kDa
PARP Cell Signaling 9542 Rabbit 1:1000 89/116 kDa
p-Bad Cell Signaling 9296 Mouse 1:1000 23 kDa
p21 Cell Signaling 2946 Mouse 1:1000 21 kDa
PTEN Cell Signaling 9552 Rabbit 1:1000 54 kDa
ER-α R&D System MAB57151 Mouse 1:2000 65 kDa
ER-β Santa Cruz sc-8974 Rabbit 1:200 56 kDa
SRC-1 BD Biosciences 612378 Mouse 1:250 165 kDa
SRC-2/TIF2 BD Biosciences 610984 Mouse 1:250 160 kDa
SRC-3/AIB-1 BD Biosciences 611105 Mouse 1:250 160 kDa
GAPDH Cell Signaling 2118 Rabbit 1:1000 38 kDa
Secondary Antibodies
Antibody Name Supplier Product Number Source Dilution  
Anti-Rabbit IgG HRP R&D System HAF008 Goat 1:2000  
Anti-Mouse IgG HRP R&D System HAF007 Goat 1:2000