Table 2 Primary and secondary antibodies for immunoblot analysis
Primary Antibodies | |||||
|---|---|---|---|---|---|
Antibody name | Supplier | Product number | Source | Dilution | Molecular weights |
Akt-1 | Cell Signaling | 2938 | Rabbit | 1:1000 | 60 kDa |
Akt-2 | Cell Signaling | 5239 | Mouse | 1:1000 | 60 kDa |
Akt-3 | Cell Signaling | 3788 | Rabbit | 1:1000 | 60 kDa |
p-Akt | Cell Signaling | 4060 | Rabbit | 1:2000 | 60 kDa |
Cleaved Caspase-3 | Cell Signaling | 9661 | Rabbit | 1:1000 | 17/19 kDa |
Cleaved Caspase-9 | Cell Signaling | 9507 | Rabbit | 1:1000 | 17 kDa |
PARP | Cell Signaling | 9542 | Rabbit | 1:1000 | 89/116 kDa |
p-Bad | Cell Signaling | 9296 | Mouse | 1:1000 | 23 kDa |
p21 | Cell Signaling | 2946 | Mouse | 1:1000 | 21 kDa |
PTEN | Cell Signaling | 9552 | Rabbit | 1:1000 | 54 kDa |
ER-α | R&D System | MAB57151 | Mouse | 1:2000 | 65 kDa |
ER-β | Santa Cruz | sc-8974 | Rabbit | 1:200 | 56 kDa |
SRC-1 | BD Biosciences | 612378 | Mouse | 1:250 | 165 kDa |
SRC-2/TIF2 | BD Biosciences | 610984 | Mouse | 1:250 | 160 kDa |
SRC-3/AIB-1 | BD Biosciences | 611105 | Mouse | 1:250 | 160 kDa |
GAPDH | Cell Signaling | 2118 | Rabbit | 1:1000 | 38 kDa |
Secondary Antibodies | |||||
Antibody Name | Supplier | Product Number | Source | Dilution | |
Anti-Rabbit IgG HRP | R&D System | HAF008 | Goat | 1:2000 | |
Anti-Mouse IgG HRP | R&D System | HAF007 | Goat | 1:2000 | |