Figure 2
From: Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells
Changes of ADHFE1 methylation and expression by treatment with 5-aza-dC in CRC cells and normal colon cells. After treatment with 5-aza-dC in cells, the methylation status and expression levels of ADHFE1 are observed using QMSP, real-time PCR and immuno-blotting analysis. A. ADHFE1 is hypermethylated in 4 CRC cells compared to normal colon cells and significantly demethylated in 4 CRC cells by 5-aza-dC. B. The mRNA expression of ADHFE1 is reduced in 4 CRC cells but increased in all cells treated with 5-aza-dC. C. The protein expression of ADHFE1 is not affected by 5-aza-dC in CCD18Co. D. Protein expression of ADHFE1 is restored by 5-aza-dC in 4 CRC cells. Expression of ACTB and GAPDH is used as a loading control. *p-Values of < 0.05 were considered statistically significant. +: Treated with 5-aza-dC; −: Non-treated with 5-aza-dC; PMR: Percentage of methylated reference.