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Table 1 Primer sequences for semi-quantitative RT-PCR

From: Suberoylanilide hydroxamic acid, an inhibitor of histone deacetylase, suppresses vasculogenic mimicry and proliferation of highly aggressive pancreatic cancer PaTu8988 cells

Gene name (mRNA ID)

Primer name

Primers sequences (5’-3’)

PCR product size (bp)

β-actin

Forw

AAGAGCTACGAGCTGCCTGACG

420

(NM_001101.3)

Rev

CGCCTAGAAGCATTTGCGGTGG

Cyclin D1

Forw

CATCTCTGTACTTTGCTTGCTCAT

499

(NM_053056.2)

Rev

CGCTATTTCCTACACCTATTGGAC

Cyclin B1

Forw

TCAACATGGCAGGCGCAAAGC

224

(NM_031966.3)

Rev

TGGCACTGGCACCAGCATAGGT

CDK1

Forw

TGCTGGGGTCAGCTCGTTACTCA

232

(NM_001786.4)

Rev

TGGGATGCTAGGCTTCCTGGTT

p53

Forw

GGGAGTAGGACATACCAGCTTAGAT

452

(NM_000546.4)

Rev

TTAGGTACTAAGGTTCACCAAGAGG

p21

Forw

CTGCCTTAGTCTCAGTTTGTGTGT

412

(NM_000389.3)

Rev

CAAAGTGCCATCTGTTTACTTCTC

CDK4

Forw

CTTGATCTGAGAATGGCTACCTCT

409

(NM_000075.2)

Rev

CATGAAGGAAATCTAGGCCTCTTA

MAGEF1

Forw

GGGTATCCGAAGAGGCTTATTATGG

749

(NM_022149.4)

Rev

AAGCAAATGAAGGTACATGCCAGTC

FGFR1

Forw

GAGGAGAAAGAAACAGATAACACCA

427

(NM_023108.2)

Rev

TGTACACCTTACACATGAACTCCAC

HIF1A

Forw

GTAAGAAGGCAGTAACCTTTCATCA

502

(NM_001530.3)

Rev

AGGGTAGGCAGAACATTTAGGTTTA

RUNX1

Forw

AGATTTAATGACCTCAGGTTTGTCG

328

(NM_001754.4)

Rev

GACTCTGAGGCTGAGGGTTAAAG

Sema4D

Forw

TGTCTGTGGAGTATGAGTTTGTGTT

552

(NM_006378.3)

Rev

GGGTGTAGTTCACATCTTTCTTGAT

VEGFA

Forw

AGAAGAGACACATTGTTGGAAGAAG

439

(NM_003376.4)

Rev

CGGTACAAATAAGAGAGCAAGAGAG

MAGED1

Forw

TCGGTCTCCTCTTGGTGATTCTGG

200

(NM_006986.3)

Rev

GTTGCTGTTGGGCACTCGTCTGT

Intergrin β5

Forw

TGCCATGCAGGTTACATCGG

353

(NM_002213)

Rev

ATCATGACGCAGTCCTTGGC

WIPF2

Forw

CTCCTTTACTCAGACATAGAGCATCA

484

(NM_133264.4)

Rev

AGACCACCCAATCTTCCACGAA

ZFP106

Forw

CCCACCTCTCACCCCTATTTCCTA

280

(NM_022473.1)

Rev

AACACCTCACCTACCCGTTTCCTG

 
  1. Note: Forw: the forward primer; Rev: the reverse primer; CDK1: cyclin-dependent kinase 1; CDK4: cyclin-dependent kinase 4; FGFR1: fibroblast growth factor receptor 1; HIF1A: hypoxia inducible factor 1, alpha subunit; MAGED1: melanoma antigen family D1; FGFR1:fibroblast growth factor receptor 1; VEGFA: vascular endothelial growth factor A; Sema4D:Sema domain, immunoglobulin domain (Ig), transmembrane domain and short cytoplasmic domain, 4D; WIPF2: WAS/WASL interacting protein family, member 2; MAGEF1: melanoma antigen family F1; MAGED1: melanoma antigen family D1; ZFP106: zinc finger protein 106 homolog; RUNX1: runt-related transcription factor 1.