Figure 5

SAHA suppresses PaTu8988 cell vasculogenic mimicry ( VM ). The six human pancreatic cancer cells including Bxpc-3, Aspc-1, CFPAC-1, PaTu8988, SW1990 and Panc-1 were harvested and suspended in RPMI-1640 plus 10% FBS at a density of 2 × 10⁵/mL. A volume of 150 μL/well of Matrigel matrix was transferred to a 48-well plate at 37°C for 30 min. Then the cells were transferred to each well. The cells were further incubated at 37°C, 5% CO₂ for 4–6 h, the formed tubes were stained with Wright-Giemsa and photographed by OLYMPUS FSX-100 microscope (A). PaTu8988 cells were treated with the indicated concentration of SAHA for 24 h, and subjected to a tube formation assay as described (B). The capillary-like structures were imaged and analyzed for quantification (C). The mRNAs of vasculogenic and angiogenic genes were determined by semi-quantitative RT-PCR assay (D), and the expression of Sema-4D was detected by western-blot (E). Experiments in this figure were repeated three times, and similar results were obtained. The data in this figure was expressed as mean ± S.E. **P < 0.01 vs. Ctrl (C). Magnification: 1:200 (A and B).