Figure 3

SAHA induces both apoptotic and non - apoptotic death of PaTu8988 cells . PaTu8988 cells were incubated with SAHA at indicated dosage for 48 h, FITC-annexin V and PI stained cells were sorted by flow cytometry (A). The distribution of cell apoptosis was analyzed (B). The protein expressions of PARP, cleaved-PARP, caspase-3, cleaved-caspase-3 and β-actin were detected by western blots (C). Experiments in this figure were repeated three times, and similar results were obtained. The data in this figure was expressed as mean ± S.E. *P < 0.05 vs. Ctrl, **P < 0.01 vs. Ctrl (B).