SAHA affects cell cycle progression of PaTu8988 cells
. PaTu8988 cells were incubated with SAHA at indicated dosage for 48 h, DNA content of PI-stained cells was analyzed by flow cytometry (A), and cell cycle distribution was analyzed (B). The mRNAs of cell cycle regulatory genes were detected by semi-quantitative RT-PCR assay (C). The protein expressions of cyclin-D1, p21, p27 and β-actin were also tested by western blots (D), and the expression and sub-cellular location of p21 were also measured by immuno-fluorescence (E). Experiments in this figure were repeated three times, and similar results were obtained. The data in this figure was expressed as mean ± S.E. *P < 0.05 vs. Ctrl, **P < 0.01 vs. Ctrl (B). Magnification: 1:400 (E).