SAHA inhibits the proliferation of PaTu8988 pancreatic cancer cells
. Chemical structure of SAHA is shown in (A). Human pancreatic cancer PaTu8988 cells were incubated with SAHA at indicated dosage for 48 h, and cell growth was measured by trypan blue exclusion test. The IC-50 was calculated by SPSS 16.0 software (B). PaTu8988 cells were treated with SAHA (20 μM). The colony formation assay was performed, and the number of colonies was manually counted (C and D). Human hypertrophic scar fibroblasts (HSF) were treated with various concentration of SAHA for 48 h, followed by Wright-Giemsa staining and photograph by OLYMPUS FSX-100 microscope (E). Peripheral blood mononuclear cells (PBMNCs) were treated with various concentration of SAHA for 48 h, cell death was detected by PI staining through FACS sorting (F). Experiments in this figure were repeated three times, and similar results were obtained. The data in this figure was expressed as mean ± S.E. **P < 0.01 vs. Ctrl (C). Magnification: 1:100 (E).