Figure 3
The effects of docetaxel treatment on the expression and localization of various ABC proteins in selected MCF-7 cell lines. (A) The effects of docetaxel on the expression of ABC proteins by immunoblotting. MCF-7CC, MCF-7TXT and MCF-7DOX cells were treated with docetaxel at indicated concentration for 24 hours. The cells were then lysed and expression level of ABCB1, ABCC1 and ABCG2 in the cells was examined by immunoblotting with specific antibodies. (B) The effects of docetaxel on the expression of ABC proteins by indirect immunofluorescence. MCF-7CC, MCF-7TXT and MCF-7DOX cells were seeded on coverslip and treated with docetaxel of indicated concentrations for 24 hours. The localization and expression of ABCB1, ABCC1 and ABCG2 in the cells were examined by indirect immunofluorescence. The green indicates the ABC proteins and the blue is the DAPI stain for nucleus. Size bar: 10 μm.