Figure 2
The expression and localization of ABC proteins. (A) The expression of various ABC proteins including ABCB1, ABCC1 and ABCG2 in MCF-7CC, MCF-7TXT and MCF-7DOX cells was determined by immunoblotting with specific antibodies. (B) Quantification of the data from three independent experiments as described in panel A. The intensity of the bands of ABC proteins was normalized against the intensity of the actin loading. The error bar is standard error. ** indicates that the difference is statistically significant with p < 0.01. (C) Immunofluorescence. The expression and the localization of various ABC proteins including ABCB1, ABCC1 and ABCG2 in MCF-7CC, MCF-7TXT and MCF-7DOX cells was determined by indirect immunofluorescence. The green indicates the ABC proteins and the blue is the DAPI stain for nucleus. Size bar: 10 μm.