Figure 4
From: Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice
IFN-γ response, CTL activity and Granzyme B ELISPOT assays on mouse splenocytes. Four mice per group were injected either with 5 μg protein or with 100 μg DNA per animal, respectively. (A) Ex vivo IFN-γ ELISPOT responses. Given are the means of IFN-γ secreting cells/104 splenocytes ± SEM. ZERA-16E7SH compared to ZERA-eGFP (p < 0.001) and to pTH (p < 0.001). (B) Splenocytes were tested by 51Cr-release assays after one round of in vitro re-stimulation for lysis of E7-wildtype expressing 2 F11 target cells. Data is given as mean ± SEM. ZERA-16E7SH compared to ZERA-eGFP (p < 0.001) and to pTH (p < 0.001). (C) Ex vivo Granzyme B ELISPOT responses. Four mice per group were immunized with 5 μg protein injected per animal. Given are the means of Granzyme B-secreting cells/104 splenocytes ± SEM. ZERA-16E7SH compared to ZERA-eGFP (p < 0.001) and to OVA (p < 0.001).