Figure 1
From: The nucleolar size is associated to the methylation status of ribosomal DNA in breast carcinomas
Location of the target regions selected for DNA methylation analysis within the rDNA locus. The picture reports a schematic representation of the rDNA locus and the location of the 3 target regions (RiboPromoter, 18S and 28S) that are amplified and analyzed by the MassARRAY EpiTYPER assay. Base positions are relative to the transcription starting site (+1) of rRNA primary transcript. For each amplicon the amplified strand is indicated, together with the sequence of the unconverted target region. The CpG sites whose methylation status can be assessed by the MassARRAY EpiTYPER assay are reported in bold. Abbreviations: 5’-ETS, 5’ external transcribed spacer; ITS1, Internal transcribed spacer 1; ITS2, Internal transcribed spacer 2; 3’-ETS, 3’ External transcribed spacer.