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Figure 4 | BMC Cancer

Figure 4

From: PMA synergistically enhances apicularen A-induced cytotoxicity by disrupting microtubule networks in HeLa cells

Figure 4

PMA increases apicularen A-mediated tubulin down-regulation. A. HeLa cells were exposed to 100 nM apicularen A in the presence or absence of 20 nM PMA. Ī±-tubulin protein levels were analyzed by Western blotting. B. HeLa cells were exposed to 100 nM apicularen A in the presence or absence of 20 nM PMA for 48 hours. Ī²-tubulin protein levels were analyzed by Western blotting. C. HeLa cells were exposed to 100 nM apicularen A in the presence or absence of 20 nM PMA for 48 hours. Ī±-tubulin mRNA levels were analyzed by RT-PCR. D. HeLa cells were exposed to 100 nM apicularen A in the presence or absence of 20 nM PMA. Cellular microtubule networks and nuclei were observed using an Olympus FV-500 fluorescence microscope. The quantified levels of Ī±-tubulin under each of the indicated conditions are shown in the histograms on the right. Error bars indicateā€‰Ā±ā€‰SD. *Pā€‰<ā€‰0.05; **Pā€‰<ā€‰0.01; ***Pā€‰<ā€‰0.001. E. HeLa cells were pretreated with 10Ā Ī¼M Ro31-8220 and then exposed to 100 nM apicularen A in the presence or absence of 20 nM PMA for 48 hours. Ī±-tubulin protein levels were analyzed by Western blotting.

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