Figure 3

PMA increases apicularen A-induced cell death in HeLa cells by activating PKCα. A. HeLa cells were transfected with siRNAs against PKCα, PKCβ and PKCγ, and incubated for 48 hours. PKCα, PKCβ and PKCγ protein levels were assessed by Western blotting. B. HeLa cells expressing siRNAs against PKCα, PKCβ and PKCγ were exposed to 20 nM PMA and 100 nM apicularen A for 36 hours. DNA content was analyzed by flow cytometry. Error bars indicate ± SEM. ***P < 0.001.