Figure 5

Coordinate growth of peritumoral IF and ODD lymphatic, vein, and artery triads. a-c): Normal contralateral IF vessel from a tumor-bearing mouse immunostained with CD31 (a) or 10.1.1 (b) includes small lymphatic, vessel, and artery in triad arrangement. Immunofluorescent imaging of subcutaneously injected Texas Red Dextran (c) shows Texas Red drainage through the lymphatic vessel, and circulation through the vein and artery 20 min after injection, demonstrating that all of these vessels are functional. d-f): Peritumoral IF vessel immunostained with CD31 (d) or 10.1.1 (e) identify enlarged lymphatic, vein, and artery triad. The lymphatic vessel appears to only be partially 10.1.1 antibody-positive, due to collapse of the lymphatic endothelium. Subcutaneously injected Texas Red Dextran is not detected in the draining IF lymphatic vessel (f), although other lymphatic drainage delivers Dextran to the functional vein and artery. g-i): ODD tumor vessel immunostained with CD31 (g) or 10.1.1 (h) consists of a triad of large lymphatic, vein, and artery. Texas Red Dextran injection labels the lymphatic as well as blood vessels, indicating that they are all functional. Scale bar 50 μm.