Figure 3

Enlarged and functional peritumoral SE lymphatic, vein, and artery triad. a). CD31 immunohistochemical staining identifies enlarged and abnormal triad of peritumoral SE lymphatic, vein, and artery. b). 10.1.1 immunohistochemical staining of the same vessel triad identifies the SE lymphatic vessel. c). Subcutaneously injected Texas Red Dextran circulates through the peritumoral SE lymphatic vessel and then into the SE vein and artery after 20 min. d). Red 10.1.1 immunofluorescent staining of an adjacent section identifies the lymphatic vessel shown in (c), and an additional collapsed lymphatic vessel. e). Green nuclear Prox1 immunofluorescent staining identifies the endothelium of both lymphatic vessels. f). Merging of the 10.1.1 (d) and Prox1 images (e) confirms that the 10.1.1-positive lymphatic vessels express Prox1. Scale bars 50 μm.