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Table 1 Details of the immunohistochemical procedure used to analyze the expression of the different proteins

From: A lactate shuttle system between tumour and stromal cells is associated with poor prognosis in prostate cancer

Protein Antibody Company Antibody dilution Positive control Incubation period Detection system
MCT4 sc-50329 Santa Cruz Biotechnology 1:500 Colon tumor Overnight R.T.U. Vectastain Universal Elite ABC Kit, Vector, EUA
GLUT1 ab 15309 Abcam 1:2000 Head and neck tumor 2 hours Ultravision Detection System Anti-polyvalent, HRP, Labvision Corporation, Freemont, CA
CAIX ab 15086 Abcam 1:2000 Stomach
MCT1 sc-365501 Santa Cruz Biotechnology 1:500 Colon tumor Overnight R.T.U. Vectastain Universal Elite ABC Kit, Vector, EUA
GLUT12 ab 75441 Abcam 1:500 Kidney Overnight  
LDHV ab 53010 Abcam 1:1000 Colon tumor   
PDK1 ab 110025 Abcam 1:500 Stomach   
AMACR 504R-16 Cell Marque 1:50 Kidney   
ACOX3 sc-135435 Santa Cruz Biotechnology 1:250 Liver 2 hours Ultravision Detection System Anti-polyvalent, HRP, Labvision Corporation, Freemont, CA
DBP DBP antibody was a gift from Dr. Gabriele Moller from HelmholtzZentrum mÜnchen Ready to use Kidney