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Figure 3 | BMC Cancer

Figure 3

From: Estrogen receptor α and aryl hydrocarbon receptor independent growth inhibitory effects of aminoflavone in breast cancer cells

Figure 3

AhR knockdown in MDA-MB-468 and Cal51 decreases AhR protein and expression of downstream targets . (A) Model of Tet-On doxycycline (Dox)-inducible AhR knockdown system engineered in MDA-MB-468 and Cal51 human breast cancer cell lines (MDA-MB-468shAhR and Cal51shAhR). (B) Western blot of whole cell lysate from MDA-MB-468shAhR and Cal51shAhR treated with vehicle control or 750 ng/mL Dox in the tissue culture medium for 7 days. (C) Quantitative representation of RPL13A-normalized levels of CYP1A1 gene expression in MDA-MB-468shAhR treated with vehicle control or 750 ng/mL Dox for seven days, then treated with compound for six hours. Data is presented as mean relative mRNA level ± S.D. of triplicate samples. (D) Quantitative representation of RPL13A-normalized levels of CYP1A1 gene expression in Cal51shAhR treated with vehicle control or 750 ng/mL Dox for seven days, then treated with compound for six hours. Data is presented as mean relative mRNA level ± S.D. of triplicate samples.

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