Figure 1
ERα -negative MDA-MB-468 and Cal51 human breast cancer cell lines exhibit sensitivity to AF . (A) Structure of Aminoflavone (5-amino-2-(4-amino-3-fluorophenyl)-6,8-difluoro-7-methylchromen-4-one; AF; NSC 686288). (B) GI50 (growth inhibition) mediated by AF plotted as concentration of AF in log [M] versus number of viable MDA-MB-468 cells. Cells were treated with AF for seven days. Data is presented as a 95% confidence interval of the GI50 value for AF. (C) GI50 (growth inhibition) mediated by AF plotted as concentration of AF in log [M] versus number of viable Cal51 cells. Cells were treated with AF for seven days. Data is presented as a 95% confidence interval of the GI50 value for AF. (D) MCF-7 human breast cancer cells and (E) MDA-MB-231 human breast cancer cells, which are reported to be sensitive and resistant respectively, were examined to validate the cell counting assay. Both cell lines were treated with AF for seven days.