Figure 5
From: The chick chorioallantoic membrane as an in vivo xenograft model for Burkitt lymphoma
Immunofluorescence staining of metastatic foci in experimental BL2B95 tumors. A-H: Immunofluorescence staining of cryosections from experimental BL2B95 tumors. Pictures show BL2B95 cells in lymphatic vessels of the CAM. Cells were stained with DAPI (blue in A, E), anti-HLA A,B,C (red in B, F) and anti-Prox1 (green in C, G). A, E: BL2B95 cells can be clearly discriminated due to their large nuclei. Al, allantoic epithelium. Ch, chorionic epithelium. B, F: HLA A,B,C staining of BL2B95 cells identifies human cells in the chick stroma. C, G: Prox1 staining of chick lymphatic endothelial cells. D, H: Merged pictures. Arrows show BL2B95 cells in CAM lymphatics. Scale bars = 50 μm in D, and 100 μm in H.