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Figure 3 | BMC Cancer

Figure 3

From: The chick chorioallantoic membrane as an in vivo xenograft model for Burkitt lymphoma

Figure 3

Transmission electron microscopy of BL2B95 tumors on the CAM. A: BL2B95 cells applied on the CAM form tumors infiltrated by granulocytes (black arrows). Apoptotic cells are found in the tumors (white arrows). B: Some tumor cells are phagocytized by macrophages (φ). Tumor cells are completely surrounded by pseudopodia and undergo apoptosis (left white arrow shows late stage apoptotic cell; right white arrow shows tumor cell in early apoptosis with cytosolic vacuolization). Black arrow shows a lymphocyte in close proximity to the macrophages. Insert shows higher magnification of Birbeck granules, which identify the cell in the rectangle as a dendritic cell. C: CAM stroma with lymphatic (lv) and blood vessels (bv) in the tumor periphery. The lumen of the lymphatic vessel is completely filled with BL2B95 cells and chick leukocytes; black arrows indicate granulocytes. The black rectangle indicates the area magnified in the insert, showing a macrophage with phagosomes of different maturation stages. D: Lymphatic vessel in the tumor center. The white arrow shows an apoptotic cell. The black arrow shows a granulocyte, which is magnified in the insert. The nucleus of the granulocyte is bi-lobed and the cytoplasm filled with rod-shaped granules. This characterizes the cell as a heterophilic granulocyte, which form the most abundant subgroup of granulocytes in the experimental tumors. (m = mitotically active cell, precondensed chromosomes). Scale bars (A-D) = 10 μm.

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