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Figure 5 | BMC Cancer

Figure 5

From: Tumor-associated macrophage-derived IL-6 and IL-8 enhance invasive activity of LoVo cells induced by PRL-3 in a KCNN4 channel-dependent manner

Figure 5

M2-polarized TAMs enhance the invasive activity of LoVo cells induced by PRL-3 via IL-6 and IL-8. A-B) The expression of cytokine profile of TAMs cocultured with LoVo-P cells and LoVo-C cells determined by qRT-PCR (A), and protein by western blotting (B). C) Cell invasion of LoVo-P/LoVo-C cells was evaluated after plating the cells on the upper cell culture inserts, with culture medium TAMs plated in the lower chambers in the presence of anti-IL-6/IL-8 antibody at 5 or 10 μg/ml, or an isotype-matched IgG control (IgG). D) The expression ratios for IL-6 and IL-8 in conditioned medium of TAMs wa determined by western blotting. GAPDH was used as a loading control. E) Similar to (C), LoVo-P cells were cocultured with TAMs that were untreated (Control), mock transfected (Lipofectamine 2000), or transfected with KCNN4-si. Bars correspond to the mean ± SD, *p < 0.05, **p < 0.01, compared with Control.

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