Figure 2

PRL-3 induces the expression of KCNN4 by TAMs via TNF-α. A)The expression of TNF-α in LoVo-P cells and LoVo-C cells was detected by western blot. B) TNF-α expression in the culture medium of LoVo-P and LoVo-C cells was detected by ELISA. C) Western blotting for KCNN4 in TAMs (Control) and TAMs cocultured with LoVo-C and LoVo-P cells. Bars correspond to the mean ± SD, **p < 0.01, compared with LoVo-P cells. D) TAMs were cocultured with LoVo-P cells for 0, 6, 12 h to detect the expression of KCNN4. Bars correspond to the mean ± SD, **p < 0.01, compared with TAMs cocultured for 12 h. E) TAMs were cocultured with LoVo-C cells for 0, 6, 12 h to detect the expression of KCNN4. Bars correspond to the mean ± SD, #p > 0.01, compared with TAMs cocultured for 12 h. F) Anti-TNF-α was added into the coculture system, and ELISA was used to detect the levels of TNF-α in the medium. Bars correspond to the mean ± SD, **p < 0.01, compared with LoVo-P cells without anti-TNF-α (Control LoVo-P). G) Western blot was used to detect the expression of KCNN4 of TAMs with anti-TNF-α in the coculture medium. Bars correspond to the mean ± SD, **p < 0.01, compared with Control.