Figure 1

THP-1 cells differentiate to M2 macrophages with PMA treatment. A) Normal conditions of THP-1 (left), and treated with PMA 320 nM for 6 h with addition of IL-4 20 ng/ml for 18 h (right). B) PMA/IL-4 treated THP-1 cells showed significant induction of CD68 (a marker of macrophage differentiation) and CD206 (a marker of TAMs/M2 macrophages).