Figure 2

Effect of RNPC1a on proliferation and growth of breast cancer cell lines MCF-7 and MB-231. (A) Western blot and (B) qRT-PCR were used to verify the efficiency of RNPC1a overexpression. The fold change of RNPC1a protein is shown below each lane. The intensity of the bands was determined using densitometric analysis. (C , D) The growth of cells over 6 days was measured using cell counting kit (CCK-8) assays. RNPC1a indicates RNPC1a overexpressing MCF-7 and MB-231 cells; NC indicates MCF-7 and MB-231 cells transfected with a vector-expressing GFP. The proliferation rate of MCF-7-RNPC1a and MB-231-RNPC1a was significantly decreased compared with control cells, respectively. Data were means of three separate experiments mean ± SEM, p < 0.05. (E) Western blot and (F) qRT-PCR were used to verify the efficiency of RNPC1a-knockdown. The fold change of RNPC1a protein is shown below each lane. The intensity of the bands was determined using densitometric analysis. (G, H) MCF-7-shRNPC1a and MB-231-shRNPC1a were significantly increased compared with control cells, respectively. Data were means of three separate experiments mean ± SEM,*p < 0.05, ***p < 0.001.