Figure 3

Ectopic expression of GAS5 inhibits gastric cancer cell proliferation in vitro . SGC7901 and BGC823 cells were transfected with pCDNA3.1-GAS5 vector (or empty vector), respectively. (A) MTT assay was performed to determine the proliferation of pCDNA3.1-GAS5 transfected SGC7901 and BGC823 cells. Data represent the mean ± s.d. from three independent experiments. (B) Viable cells were counted by trypan blue staining at 72 h after transfection. (C, D) Colony-forming growth assay was performed to determine the proliferation of pCDNA3.1-GAS5 transfected SGC7901 and BGC823 cells. The colonies were counted and captured. **P < 0.01.