Activation of STAT3 in response to paclitaxel treatment in HEY cells. HEY cells were treated with paclitaxel (1 ng/ml) for 6, 12, 24, 48 and 72 hours. Cell lysates were prepared and Western blot was performed as described in the Methods. Total protein loading was determined by probing the membranes for GAPDH. Results are representative of three independent experiments.