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Figure 4 | BMC Cancer

Figure 4

From: Matrix metalloproteinase-10 promotes tumor progression through regulation of angiogenic and apoptotic pathways in cervical tumors

Figure 4

MMP-10 regulates the expression of key angiogenic and metastatic factors. A, Angiogenesis RT2 Profiler PCR Arrays evaluated by quantitative RT-PCR assessed the expression of 84 gene transcripts. Gene expression levels were normalized to the combination of five housekeeping genes (β-actin, B2M, GAPDH, HPRT and RPLP). Changes in expression of HIF-1α, MMP-9, MMP-2, IGF-1 transcripts (see other changes listed in Additional file 3: Table S1) were noted in HeLa-MMP-10OE cells relative to HeLaEmpty cells. B, Western blot analysis confirmed the differential expression of HIF-1α and MMP-9 at the protein level. GAPDH served as a loading control. C, Metastasis RT2 Profiler PCR Arrays evaluated by quantitative RT-PCR assessed 84 gene transcripts. Gene expression levels were normalized to the combination of five housekeeping genes (β-actin, B2M, GAPDH, HPRT and RPLP). Changes in expression of Src, MMP-2, PAI-1, CXCR2 (also see Additional file 4: Table S2) were noted in HeLa-MMP-10OE cells relative to HeLaEmpty cells. D, Western blot analysis confirmed differential protein expression of PAI-1 and CXCR2. GAPDH served as a loading control.

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