Figure 3
MMP-10 promotes cellular migration and invasion. A, HeLa cells (parental, HeLaEmpty and HeLa-MMP-10OE) and B, UROtsa cells (parental, UROtsa-MMP-10KD-1, UROtsa-MMP-10KD-2 and UROtsa-MMP-10Scr) were subjected to in vitro migration and invasion assays. Data are average values and SDs of three independent experiments conducted in triplicate. Overexpression of MMP-10 in HeLa-MMP-10OE cells was noted to correspond with an increase in migratory and invasive potential, while silencing of MMP-10 in UROtsa-MMP-10KD-1 resulted in a reduced migratory potential. C, HUVEC cells were placed in the invasion chamber and then exposed to conditioned media in the lower chamber from HeLa cells (parental, HeLa-MMP-10OE and HeLaEmpty), and UROtsa cells (UROtsa-MMP-10KD-1, UROtsa-MMP-10KD-2 and UROtsa-MMP-10Scr) for in vitro migration and invasion assays. Data are average values and SDs of three independent experiments conducted in triplicate. Conditioned media from HeLa-MMP-10OE cells that overexpressed MMP-10 was noted to induce migration and invasion of HUVEC cells. D, Capillary tube formation of HUVECs cultured in conditioned media from HeLa-MMP-10OE and HeLaEmpty cells were quantified as total tube length per field in micrometers and was noted to be enhanced when MMP-10 was overexpressed. Data are average values and SDs of three independent experiments conducted in triplicate. E, Capillary tube formation in HUVECs cultured in conditioned media from UROtsa-MMP-10KD-1, UROtsa-MMP-10KD-2 and UROtsa-MMP-10Scr cells were quantified as total tube length per field in micrometers and was noted to be inhibited when MMP-10 was silenced. Data are average values and SDs of three independent experiments conducted in triplicate. *, p < 0.05.