Figure 3

Drug-induced irreversible differentiation of I-type NB cancer stem cell confirms the association miRNAs with cell phenotype. A. Fold increases in miR-21, -221 and -335 in BE(2)-C cells differentiated to an S phenotype following a 2 week treatment with 10^-5 M BrdU. B. Changes in miR-124 and -375 expression in BE(2)-C cells treated with RA or BrdU to induce an N or S phenotype, respectively. Each bar represents the mean ± SEM of 4–6 determinations normalized to untreated controls set =1.0.