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Table 1 Sphere-forming efficiency of the three MPM cell lines

From: Cisplatin-resistant cells in malignant pleural mesothelioma cell lines show ALDHhighCD44+ phenotype and sphere-forming capacity

Cell line

Sphere generation

Sphere size (μm)

Sphere number/SD

Sphere efficiency (%)

H28

1°

50 - 120

51/17.8

1.0

 

2°

40 - 100

47.7/4.9

0.95

 

3°

40 - 100

20/5.0

0.40

H2052

1°

100 - 600

11.5/5.7

0.23

 

2°

120 - 500

20/6.2

0.40

 

3°

100 - 500

25/7.4

0.50

Meso4

1°

80 - 600

14/5.7

0.28

 

2°

40 – 500

12.5/5.3

0.25

 

3°

40 - 500

10/5.0

0.20

  1. Single-cell suspensions of H28, H2052 and Meso4 were seeded at 5 x 103/well/ml onto a 24-well plate. Sphere-forming efficiency (%) was determined by dividing the number of spheres formed by the original seeded cells. The quotient was then multiplied by 100.