Figure 5

Cisplatin + DEAB treatment decreases cell viability and induces a short-term inhibition of sphere formation. ALDH^high- and ALDH^low-sorted cells of H28, H2052 and Meso4 were pre-treated with 100 μM DEAB for 48 h, then re-incubated with cisplatin (Cis) for either 48 h or 72 h. Cell viability of the surviving cells was quantified using tryphan blue exclusion test which was performed in triplicates in 3 independent experiments (A-C). Surviving cells were allowed to form spheres under an anchorage-independent culture condition which were evaluated on day 7 and then compared with the non-treated cells (D-F). Results represent means and SDs of 3 experiments with 6 replicates each. Dashed lines symbolize 0 values. The level of significance was set at p <0.05 (*p <0.05, **p <0.01, ***p <0.001). Representative images of sphere-formation of ALDH^high -sorted MPM cell lines, with and without cisplatin treatment, and in the presence or absence of 100 μM DEAB (G). The ALDH^low -sorted fraction of each cell line showed the same effect (images not shown).