Skip to main content
Figure 4 | BMC Cancer

Figure 4

From: Cisplatin-resistant cells in malignant pleural mesothelioma cell lines show ALDHhighCD44+ phenotype and sphere-forming capacity

Figure 4

Effect of cisplatin treatment on ALDH activity and sphere-forming efficiency. Cells in 10 cm dishes were treated with the previously determined IC50 of cisplatin for ALDH-sorted fractions of the MPM cell lines. After the 48- and 72-h cisplatin treatments of ALDHhigh- and ALDHlow-sorted cells, ALDH activity (A-C) was determined on surviving cells and compared with the non-treated cells to evaluate the effect of cisplatin by flow cytometry. The sphere-forming efficiency of the cisplatin-resistant cells was also evaluated as described in the Methods, and compared with the non-treated cells (D-F). Results represent the means and SDs of 3 independent experiments each. Data are statistically significant if p <0.05 (*p <0.05, **p <0.01, ***p <0.001). Representative images of spheres from non-treated and cisplatin-treated H2052 ALDHhigh - and H2052 ALDHlow -sorted cells (G).

Back to article page