Effect of DHT and bicalutamide alone and in combination with PI3K/mTOR, Erk1/2 and EGFR/PDGFRβ inhibitors in AR expression and cell proliferation. A) Effect of DHT and bicalutamide, BEZ and PD in alone and combined on AR expression in Hs578T and BT549. Cells were cultured and treated with drugs for 24 h. Cell lysates were analyzed by western blot for AR expression. α-tubuline was used as loading control. B) In parallel, total RNA was extracted and subjected to RT-PCR. Subsequently, amplification products were resolved on agarose gel to determine AR mRNA levels. Actin was used as protein loading control and mRNA as gene control.