Figure 4

Comparison of the influence of anti-GD2 antibodies on viability of GD2-positive vs. GD2-negative tumor cell lines. The viability of GD2-positive (EL-4, IMR-32, mS) and GD2-negative (Neuro-2A, A375, Jurkat) tumor cells was assessed for the cells incubated with various concentration of anti-GD2 mAbs for 72 h using MTT assay as described in Methods. Results are shown for two monoclonal anti-GD2 antibodies 14G2a (A) and ME361 (B). Mean ± S.E. of three separate experiments is shown, statistical analysis was performed using two-way analysis of variance method for concentrations of 0.31 – 10 μg/ml (A), and for concentrations 2.5 – 10 μg/ml (B). The differences between GD2-positive and GD2-negative groups were statistically significant (***, P < 0.001) as determined by Student-Newman-Keuls post-hoc analysis.