Expression of PAX8 induces EMT in normal ovarian cells.
(A) Quantitative PCR for Snail, Zeb2, Twist, Fibronectin, Vimentin, MMP13 and TIMP3 in IOSE80-3XFP8-IB9 and IOSE80-3XFP8-IIB5 clones (IOSE-80PC cells transfected with 3XFLAG-Pax8) and in IOSE80-Ctrl (IOSE-80PC transfected with the backbone vector). Columns, mean of three independent experiments in duplicate; bars, SD. *, Ρ < 0.01. (B) Total protein extracts of IOSE80-Ctrl cells and independent stable clones IOSE80-3XFP8-IB9 and IOSE80-3XFP8-IIB5 were separated on SDS-PAGE and subjected to Western blot analysis with specific antibodies as indicated in the panel. The hybridization with vinculin assessed the protein uniform loading and integrity.