Figure 4
From: A role for PAX8 in the tumorigenic phenotype of ovarian cancer cells
Expression of PAX8 induces EMT in normal ovarian cells. (A) Quantitative PCR for Snail, Zeb2, Twist, Fibronectin, Vimentin, MMP13 and TIMP3 in IOSE80-3XFP8-IB9 and IOSE80-3XFP8-IIB5 clones (IOSE-80PC cells transfected with 3XFLAG-Pax8) and in IOSE80-Ctrl (IOSE-80PC transfected with the backbone vector). Columns, mean of three independent experiments in duplicate; bars, SD. *, Ρ < 0.01. (B) Total protein extracts of IOSE80-Ctrl cells and independent stable clones IOSE80-3XFP8-IB9 and IOSE80-3XFP8-IIB5 were separated on SDS-PAGE and subjected to Western blot analysis with specific antibodies as indicated in the panel. The hybridization with vinculin assessed the protein uniform loading and integrity.