Loss of PAX8 inhibits anchorage-independent growth in soft agar and suppresses tumorigenesis in nude mice. (A) soft agar growth of SKOVCtrl-, and siPAX8 cells was assessed and photographed. Columns, mean of three independent experiments; bars, SD (Ρ < 0.05). (B) growth curves of tumors in tumor-bearing nude mice injected with SKOV3, SKOVCtrl-, siCl32 and siCl48 cells. Data are plotted as the mean volume ± SD (n = 6 for each group). (C) representative images of SKOV3, SKOVCtrl-, siCl32 and siCl48 tumors following surgical resection.