PAX8 silencing in SKOV-3 cells inhibits cell proliferation, migration, and invasion. (A) growth curves of SKOV-3, SKOVCtrl-, siCl32, and siCl48 cells are shown. Triplicate of 8 × 104 cells were seeded into 60-mm plate. Cell numbers were counted on days 1, 2, 3, 4, 5 and 6 after seeding. (B) wound-healing migration assay for SKOVCtrl-, siCl32, and siCl48 were performed. The healing of the wounds by migrating cells was imaged at time 0, 8 and 24 h (upper panel). Quantitation of the wound-healing migration assay. Wound closure was calculated as the distance covered by cells in relation to the initial wound diameter, as determined at 0 h. Wound closure is expressed as the percentage of the initial wound diameter at 0 h. Data are expressed as the mean ± SD (Ρ < 0.01, bottom panel). (C) Matrigel invasion assay of SKOVCtrl-, and siPAX8 clones is shown. Columns, mean of three independent experiments; bars, SD (Ρ < 0.05).