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Figure 3 | BMC Cancer

Figure 3

From: Role of malignant ascites on human mesothelial cells and their gene expression profiles

Figure 3

TRAIL-induced apoptosis in ascites-stimulated HPMCs. (A) Diagram of HPMC-priming assays. Ascites-stimulated or benign fluid-stimulated HPMCs were culture overnight (shown in yellow), washed TWICE and cultured in serum/hormone-free medium for 8 to 24 h to generate HPMC-conditioned medium (shown in pink) that were collected at either 12 h (B) or different time points (C). HPMC-conditioned medium was then added to CaOV3 tumor cells in the presence of TRAIL (25 ng/ml). TRAIL-induced apoptosis was measured in CaOV3 cells incubated with the indicated HPMC-conditioned medium overnight and expressed as fold increased relative to cells that were exposed to HPMC-conditioned medium but not to TRAIL. Data are expressed as means of triplicates from three independent experiments ± SD. * indicate P < 0.01.

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