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Figure 5 | BMC Cancer

Figure 5

From: N-nitroso-N-ethylurea activates DNA damage surveillance pathways and induces transformation in mammalian cells

Figure 5

NEU induces upregulation of vimentin and disrupts polarity in MCF10A breast acini. MCF10A cells were grown as 3D ‘on top’ cultures in Matrigel™. 2, 3 and 5 mM NEU was administered on Day 0 and Day 2. The acini were cultured for 20 days and then immunostained for (A) α6-integrin (green), β-catenin (red) and DAPI (blue) to stain nuclei (B) Vimentin (green) a marker for EMT and (C) E-cadherin (green). The data is representative of 40 – 50 acini from three biologically independent experiments.

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