Retroviral-induced EGFR over expression in MCF7 human breast cancer cells enhances downstream signalling
. EGFR expression was determined in parental MCF7 and MCF7-EGFR cells by immunofluorescence (A) and FACS analysis (B). To determine downstream EGFR signalling, starved MCF7 parental and MCF7-EGFR cells were stimulated with EGF (100 ng/mL). Cell lysates were collected and analyzed by western blot for the phosphorylation status of EGFR, MAPK1/3 and Akt as well as the expression of ERα (C).