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Figure 1 | BMC Cancer

Figure 1

From: Epidermal growth factor receptor signalling in human breast cancer cells operates parallel to estrogen receptor α signalling and results in tamoxifen insensitive proliferation

Figure 1

Retroviral-induced EGFR over expression in MCF7 human breast cancer cells enhances downstream signalling . EGFR expression was determined in parental MCF7 and MCF7-EGFR cells by immunofluorescence (A) and FACS analysis (B). To determine downstream EGFR signalling, starved MCF7 parental and MCF7-EGFR cells were stimulated with EGF (100 ng/mL). Cell lysates were collected and analyzed by western blot for the phosphorylation status of EGFR, MAPK1/3 and Akt as well as the expression of ERα (C).

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BMC Cancer

ISSN: 1471-2407

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