Figure 3From: RETRACTED ARTICLE: STMN-1 is a potential marker of lymph node metastasis in distal esophageal adenocarcinomas and silencing its expression can reverse malignant phenotype of tumor cells Verification of knockdown of STMN1 gene expression in esophageal cancer cell line by lentivirus-mediated RNA interference. (A) Western blot analysis of STMN1 protein in lysates of either untreated (control) or transfected with a Non-silencing shRNA (scrambled sequence) and transfected with a specific STMN1 shRNA. ACTIN expression was used as a loading control. (B) Densitometry analysis of A normalized to Actin, Laser densitometric analysis of protein bands was performed, and the ratio of stathmin expression to Beta-Actin expression (STMN:Beta-Actin) was determined and normalized. (C) Total RNA isolated from distal esophageal adenocarcinoma cells before and after silencing stathmin gene expression using STMN1 shRNA were analyzed with real-time RT–PCR. There was no significant difference between controls and non-silencing shRNA (scrambled sequences). Data are expressed as percentage change (Means ± S.D.) compared with controls and represent four independent experiments. (P < 0.05 vs Non-silencing shRNA, one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparion).Back to article page