Figure 6From: Breast tumor specific mutation in GATA3 affects physiological mechanisms regulating transcription factor turnover Biochemical characterization of wild-type and mutant GATA3 using a nucleic acid analog. Nuclear extracts were prepared from A) T47D and B) MCF7 cells and applied to heparin sepharose. The column was eluted with linear gradient of NaCl concentration from 0.1 to 1Â M. The separated fractions were analyzed by Western blot directed against GATA3.Back to article page