Figure 5

DNA binding ability of wild-type and mutated GATA3. Electrophoretic Mobility Shift Assay (EMSA) was performed using recombinant A) wild-type GATA3 DNA binding domain (DBD) or B) mutated GATA3-DBD. Equivalent molar amounts of wild-type and mutant protein, assessed by Coomassie blue stained SDS-PAGE, were used. C) In a competitive assay, an equimolar mix of wild-type and mutated DBD proteins was used. To test protein ability to bind DNA, GATA3 motif-containing oligonucleotides (GATA +) and GATA3 motif-lacking oligonucleotides (GATA-) were used. WT – wild-type, Mut – mutated GATA3.