Cellular localization of wild-type and mutated GATA3 in breast cancer cells. A) Immunofluorescence staining of T47D and MCF7 cells. Scale bar corresponds to 20 μm. B) Subcellular fractionation of T47D or MCF7 cell extracts in 0.1-0.8 M NaCl. Input – whole cell lysate, CF – cytoplasmic fraction, NE – nuclear extract.