Figure 7

Expressions of Sp1 and p65 were regulated by E2F1. (A) Immunohistochemistry staining of Sp1, p65, and E2F1 (1:50 antibody dilution) were positive in the sections of SCLC tissue where MMP-9 was positive (Scale bar = 50 μm). (B) The expressions of Sp1 and p65 were significantly decreased in E2F1i group as compared with Mock and NCi by real time PCR and western blotting (*P < 0.05). (C) E2F1 could induce the activity of Sp1 promoter, E2F1 binding site mutant 1 and 2, but not mutant 3 (*P < 0.05). (D) E2F1 could enhance the activity of p65 promoter, but not E2F1 binding site mutant 1 and 2 (*P < 0.05).