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Figure 6 | BMC Cancer

Figure 6

From: Differential regulation of MMPs by E2F1, Sp1 and NF-kappa B controls the small cell lung cancer invasive phenotype

Figure 6

Expressions of MMP-9 was regulated by Sp1 and NF-kappaB. (A) Transient transfection experiments in H1688, H446 and A549 cells showed that E2F1 could enhance the activity of MMP-9 promoter and E2F1 binding site mutant promoter (*P < 0.05). (B) Sp1 could enhance the activity of MMP-9 promoter, Sp1 binding site mutant 1 and 2, but not mutant 3 and 4 (*P < 0.05). (C) p65 could significantly induce the activity of MMP-9 promoter, but not NF-kappaB binding site mutant 1 and mutant 2 (*P < 0.05). (D) Real time PCR and western blotting results showed that the expression of MMP-9 was significantly decreased when Sp1 or p65 were interfered by specific siRNA (Sp1i or p65i) as compared with untreated (Mock) and scramble siRNA (NCi) (**P < 0.01).

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