Figure 5

E2F1 regulated the expression of MMP-16. (A) Immunohistochemistry staining showed that MMP-16 was strong staining in SCLC sample, where E2F1 was positive expression. (the upper panel scale bar = 100 μm; the below panel scale bar = 50 μm). (B) E2F1 was overexpressed in A549 cells transfected with pcDNA3.1-E2F1 vector as compared with the untreated (Mock) and vehicle (pcDNA3.1). (C) Transient transfection experiments showed that E2F1 could significantly induce the activity of MMP-16 promoter, E2F1 binding site mutant 1, but not E2F1 binding site mutant 2 (*P < 0.05). RLA represents relative luciferase activity.