GRΔLBD activates the
promoter in the presence and absence of HC. EPH-4 cells were transiently transfected with the Ch25h promoter reporters A. Ch25h-11.5-pRL and B. Ch25h-12-pRL as well as with expression vectors for GABPα/β (GABP), full-length GR (GR FL) and GR lacking the ligand binding domain (GRΔLBD). Cells were treated 24 hours after transfection with either ethanol vehicle (-HC) or 1 μg/mL HC (+HC) and assayed for luciferase activity following a 48 hour incubation. Bars represent the mean of technical replicates, and error bars represent standard deviation (N = 3). Statistically significant changes in Ch25h promoter activity relative to the EV (-HC) transfection are indicated: one asterisk, p < 0.05 (significant); two asterisks, p < 0.005 (very significant).