Figure 4
From: Inhibition of autophagy enhances the cytotoxic effect of PA-MSHA in breast cancer
Protective effects of autophagy. A. Cells treated by 3-MA (2 mM), PA-MSHA (10 × 108/ml) or PA-MSHA together with 3-MA for 48 hours were visualized by light microscopy. Most cellular vacuolization and death were found in cells treated with PA-MSHA and 3-MA. B. The percentage of apoptotic cells in MDA-MB-231-shIRE1, MDA-MB-231HM-shIRE1 cells, MDA-MB-468 cells was measured by flow cytometric analysis. Cells were treated by 3-MA (2 mM), PA-MSHA (10 × 108/ml) or PA-MSHA together with 3-MA for 48 hours (*P < 0.05). C. The viability of cells treated by PA-MSHA (10 × 108 cells/ml), 3-MA (2 mM) or PA-MSHA together with 3-MA was evaluated by Cell Counting Kit-8 assay. D &E The expression of caspase3, cleaved-caspase3 and LC3 in cells treated with PA-MSHA (10 × 108 cells/ml), PBS, 3-MA (2 mM) or PA-MSHA together with 3-MA (D). The relative expression of cleaved-caspase3 and LC3-II/LC3-I of each sample.